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Microarray analysis and validation of KDM4D mRNA expression in bMECs stimulated with Mycoplasma bovis ; bovine mammary epithelial cells (bMECs) were incubated with live M. bovis at an multiplicity of infection of 1,000 for 6 h. The data are expressed from three (microarray) or four (real-time polymerase chain reaction [PCR]) independent experiments. Significant difference ( * p <0.05) compared with unstimulated bMECs.

Journal: Journal of Veterinary Science

Article Title: Innate immune response of bovine mammary epithelial cells to Mycoplasma bovis

doi: 10.4142/jvs.2018.19.1.79

Figure Lengend Snippet: Microarray analysis and validation of KDM4D mRNA expression in bMECs stimulated with Mycoplasma bovis ; bovine mammary epithelial cells (bMECs) were incubated with live M. bovis at an multiplicity of infection of 1,000 for 6 h. The data are expressed from three (microarray) or four (real-time polymerase chain reaction [PCR]) independent experiments. Significant difference ( * p <0.05) compared with unstimulated bMECs.

Article Snippet: The gene expression dataset was obtained via Agilent single-color microarray platform (4 × 44K bovine gene expression array, grid ID 023647).

Techniques: Microarray, Expressing, Incubation, Infection, Real-time Polymerase Chain Reaction